DNA Isolation Kits

For Simple and Fast Extraction of Genomic DNA

With DNA isolation kits you extract DNA from bacteria, yeasts or molds from enrichments or production samples. We develop the kits together with the later users and individually adapt them for both the material to be analysed and the composition of the potential spoilers which are expected. DNA isolation kits are available in different varieties. Besides the ones which are described in the following, we also offer PIKA ColumnEx (DNA cleaning) and PIKA SeedEx (grain analysis).

The PIKA Approach

For us it is particularly important that our customers are able to work without major instrumental or time investments. Our DNA Isolation Kits are simple and robust, suitable for use in practical applications. The isolated DNA can be further used in different molecular biology based examinations: For detection of microorganisms as well as for the detection of contaminations with other species or with genetically modified material.

Simple Use

With a few centrifugation and pipetting steps from sample to DNA: With our easy to use DNA Isolation Kits even non-molecular biologists can process their samples for PCR analysis without problems. We make your work so easy.

Universal, Robust

Our DNA Isolation Kits are suitable for use in the production lab, we have one procedure for all issues. Beer samples and yeast containing process samples are treated with the same method – afterwards the DNA can be examined for bacteria and yeasts.

Fast, Cost Efficient

We keep an eye on both your laboratory and your personal capacities. Sample processing with our DNA Isolation Kits takes 15 minutes only. The equipment needed is consisting of pipets, a bench centrifuge and a Vortexer or heating block/water bath only.

PIKA BeadEx – for Fast DNA Isolation from Bacterian

The DNA Isolation Kit PIKA BeadEx is intended for fast DNA extraction from bacteria coming from enrichments. Bacteria can be taken from solid surfaces (e.g. colonies from membrane filters) or from liquid samples. Cell disruption is achieved mechanically without costly instruments by shaking in the presence of glass beads. The isolated DNA can be further used in diverse molecular biology analyses as PCR.

Advantages:

  • Different samples, one procedure
  • Very fast and straightforward
  • Also suited for microorganisms with durable cell walls – Alicyclobacillus
  • No specialists necessary
  • Without organic solvents
  • No hazardous waste

Fields of application:

  • Routine control and quantification in breweries and wineries
  • Identification and verification of positives
  • Routine control in juice and concentrate production
  • Alicyclobacillus monitoring according to IFU No 12- the total analysis time is shortened 5 days

PIKA HeatEx – for Beer, Yeast Containing Samples, Food and Beverages

Fast DNA extraction from enriched samples: PIKA HeatEx DNA Isolation Kits are optimized for the examination of beer and yeast containing production samples as well as from sugar containing food and beverages. With one single extraction genomic DNA is isolated both from bacteria and yeast. The samples can be taken from solid surfaces (e.g. colonies from membrane filters) or from liquid samples. Cell disruption is achieved thermally by heating in the presence of lysis buffer. The isolated DNA can be further used in diverse molecular biology analyses as PCR.

Advantages:

  • DNA from bacteria and yeasts with one procedure
  • Very fast and straightforward
  • Minimal inhibition risk in PCR
  • Different samples, one procedure
  • No specialists necessary
  • Without organic solvents
  • No hazardous waste

Fields of application:

  • Monitoring of microbiological status in all areas
  • Routine control and quantification in breweries and wineries
  • Identification and verification of positives
  • Routine control in juice and concentrate production

Further PIKA Products and Services

Competence in microbiology with a broad background: The innovative products from PIKA are derived from applications of our own in-house analytics and services for producers. All our products from the whole portfolio are perfectly matched. PIKA products are outstanding in easy handling and extreme reliability, our services in short processing time and top quality at affordable prices.

PCR kits are all-inclusive

PIKA was first to come to market with PCR kits in a dried format. Each kit is a complete package including material for DNA extraction as well as reagents and tubes for PCR thus covering the whole analysis. All product relevant microorganisms are detected within one single test, the handling is quite simple.

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FastOrange Enrichment Media

Developed by us to avoid microbiological damage of beer and many other beverages: PIKA FastOrange can be used by every brewer and every beverage producer for routine  control throughout the whole process line. FastOrange is available in different varieties, according to the capabilities and equipment of your own lab.

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Microbiology Analyses

We are fast and competent in doing routine analyses as enrichments, determination of colony counts and the differentiation of microorganisms – service and quality are most important for us. Of course we respond to your requirements and always deliver prompt and reliable results – if necessary, even through weekends.

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Broad Process Consultancy

Our work does not end at the analysis result, we further advise you how to deal with a contamination and propose the next steps to minimize the risk for further batches in your production line. You can also count on us when optimizing your routine quality control measures.

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PIKA Weihenstephan – your partner in microbiology for beverage production
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