Our PCR kits are intended for microtiter plate format and can be used with real time PCR thermocycler instruments from different manufacturers. They were developed for use in the quality control laboratory of a producing company. You don’t have to be a specialist to use our special analytics and gain reliable results. No prior knowledge is needed. PCR material can be used separately for each sample so no reaction is lost.
All PIKA Detection Kits are available in two formats so they can be used in every common real time PCR thermocycler instrument. Generally you can use our dried PCR mixes without modifications in any cycler with a thermoblock in microtiter plate format. Corresponding to the shape of thermoblocks from different thermocycler manufacturers, different PCR tubes have to be used. Accordingly we offer two PCR kit series, TM Detection Kits and SO Detection Kits. TM Detection Kits are containing 0.2 mL PCR tubes which are a bit higher, while the PCR tubes in the SO Detection Kits have a capacity of 0.1 mL.
For other thermocyclers with different tube formats as capillaries, the PCR reagents can be transferred into the proprietary tubes of the instrument manufacturer. In this case we recommend the use of SO Detection Kits.
You find a list of thermocyclers and the corresponding PIKA Detection Kits here.
|Manufacturer||real time PCR
PCR Detection Kit type
|Applied Biosystems (Thermo Fisher Scientific)||StepOne™||SO|
|Applied Biosystems (Thermo Fisher Scientific)||StepOne Plus™||SO|
|Applied Biosystems (Thermo Fisher Scientific)||ABI PRISM® 7000||TM|
|Applied Biosystems (Thermo Fisher Scientific)||Applied Biosystems 7300||TM|
|Applied Biosystems (Thermo Fisher Scientific)||Applied Biosystems® 7500||TM|
|Applied Biosystems (Thermo Fisher Scientific)||Applied Biosystems® 7500 Fast||SO|
|Applied Biosystems (Thermo Fisher Scientific)||QuantStudio® 3||TM|
|Applied Biosystems (Thermo Fisher Scientific)||QuantStudio® 3 Fast||SO|
|Applied Biosystems (Thermo Fisher Scientific)||QuantStudio® 5||TM|
|Applied Biosystems (Thermo Fisher Scientific)||QuantStudio® 5 Fast||SO|
|Manufacturer||real time PCR thermocycler instrument||PIKA Weihenstephan recommended PCR Detection Kit type||Adaption required|
|Bio Rad||CFX96 Touch™||TM|
|Bio Rad||CFX Connect™||TM|
|Bio Rad||iCycler™||SO||Has to be pipetted into PCR tubes recommended by manufacturer.|
|Bio Rad||iQ™4||SO||Has to be pipetted into PCR tubes recommended by manufacturer.|
|Bio Rad||iQ™5||SO||Has to be pipetted into PCR tubes recommended by manufacturer.|
|Bio Rad||MyiQ™||SO||Has to be pipetted into PCR tubes recommended by manufacturer.|
|Bio Rad||MyiQ2™||SO||Has to be pipetted into PCR tubes recommended by manufacturer.|
|Eppendorf||Mastercycler® EP Realplex||TM|
|Qiagen||Rotor-Gene Q||SO||Has to be pipetted into PCR tubes recommended by manufacturer.|
|Roche||Light Cycler® 96||SO||Adaptor and Calibration file required.|
|Roche||Light Cycler® 480||SO||Adaptor for instrument required, older instrument version requires calibration file.|
|Roche||Light Cycler® Nano||SO|
|Thermo Fisher Scientific||PikoReal™||SO||Has to be pipetted into PCR tubes recommended by manufacturer.|
|Thermo Fisher Scientific||ProFlex™||TM|
|IT-IS Life Science Ltd||MyGo!||SO|
|IT-IS Life Science Ltd||MyGo! Mini||SO||Has to be pipetted into PCR tubes recommended by manufacturer.|
PIKA Detection Kits B were developed for the routine quality control of products and the production line. Different groups of microorganisms which are spoiling a product are specifically detected together by screening kits. A simple yes/no analysis covering all spoilage bacteria in one single test allows the fast and reliable control of the whole production line – and even the costs stay within reasonable limits. Further differentiation is achieved by identification kits which are available for a variety of bacteria species.
PCR detection Kits H are optimized for the examination of beer and sugar containing food and beverages. The complete spectrum of yeasts and molds can be tested with one single analysis – free of yeasts and molds yes/no? Different yeasts which may be critical in different products as beer and wine are detected with specific screening or identification kits.
PIKA Detection Kits S were developed for easy differentiation of molds. If molds are appearing in a process step or in the finished product, the question about their heat resistance is crucial for the further processing. With PCR detection kits, heat resistant molds are clearly identified. Our kits are also in use for the detection of mycotoxin producing Fusarium species on malt, grain and cereals as well as in flour.
Developed by us to avoid microbiological damage of beer and many other beverages: PIKA FastOrange can be used by every brewer and every beverage producer for routine control throughout the whole process line. FastOrange is available in different varieties, according to the capabilities and equipment of your own lab.
Developed for use in a production laboratory: Optimised for the extraction of genomic DNA from beer, wine and other beverages as well as from malt, grain and cereals – for the detection of bacteria, yeasts and molds. Cost saving thanks to simple and fast handling and a minimum of equipment needs.
Spoilt product or positive enrichments are further discriminated – fast and specific by PCR analyses the same day as the samples are received. We further identify unknown bacteria, yeasts or molds from product or the production area within 3 to 5 days by DNA sequencing.
Our work does not end at the analysis result, we further advise you how to deal with a contamination and propose the next steps to minimize the risk for further batches in your production line. You can also count on us when optimizing your routine quality control measures.