PCR Kits

For Fast Detection of Product Spoilers

PIKA Weihenstephan is offering PCR kits in dried format already since the company funding in the year 2000. We were first in the market. Each sales unit is a complete package ready to use: It is consisting of material for DNA isolation as well as of reagents and plastics for PCR, thus covering the whole analysis.

The PIKA Approach

Our PCR kits are intended for microtiter plate format and can be used with real time PCR thermocycler instruments from different manufacturers. They were developed for use in the quality control laboratory of a producing company. You don’t have to be a specialist to use our special analytics and gain reliable results. No prior knowledge is needed. PCR material can be used separately for each sample so no reaction is lost.

PIKA Detection Kits for Every real time PCR Thermocycler

All PIKA Detection Kits are available in two formats so they can be used in every common real time PCR thermocycler instrument. Generally you can use our dried PCR mixes without modifications in any cycler with a thermoblock in microtiter plate format. Corresponding to the shape of thermoblocks from different thermocycler manufacturers, different PCR tubes have to be used. Accordingly we offer two PCR kit series, TM Detection Kits and SO Detection Kits. TM Detection Kits are containing 0.2 mL PCR tubes which are a bit higher, while the PCR tubes in the SO Detection Kits have a capacity of 0.1 mL.
For other thermocyclers with different tube formats as capillaries, the PCR reagents can be transferred into the proprietary tubes of the instrument manufacturer. In this case we recommend the use of SO Detection Kits.

You find a list of thermocyclers and the corresponding PIKA Detection Kits here.

Manufacturer real time PCR
thermocycler instrument
PIKA Weihenstephan
recommended
PCR Detection Kit type
Adaption required
Agilent Technologies MX4000® TM
Agilent Technologies MX3000P® TM
Agilent Technologies MX3005P™ TM
Applied Biosystems (Thermo Fisher Scientific) StepOne™ SO
Applied Biosystems (Thermo Fisher Scientific) StepOne Plus™ SO
Applied Biosystems (Thermo Fisher Scientific) ABI PRISM® 7000 TM
Applied Biosystems (Thermo Fisher Scientific) Applied Biosystems 7300 TM
Applied Biosystems (Thermo Fisher Scientific) Applied Biosystems® 7500 TM
Applied Biosystems (Thermo Fisher Scientific) Applied Biosystems® 7500 Fast SO
Applied Biosystems (Thermo Fisher Scientific) QuantStudio® 3 TM
Applied Biosystems (Thermo Fisher Scientific) QuantStudio® 3 Fast SO
Applied Biosystems (Thermo Fisher Scientific) QuantStudio® 5 TM
Applied Biosystems (Thermo Fisher Scientific) QuantStudio® 5 Fast SO
Manufacturer real time PCR thermocycler instrument PIKA Weihenstephan recommended PCR Detection Kit type Adaption required
Bio Rad CFX96 Touch™ TM
Bio Rad CFX384™ TM
Bio Rad CFX Connect™ TM
Bio Rad Opticon™ TM
Bio Rad iCycler™ SO Has to be pipetted into PCR tubes recommended by manufacturer.
Bio Rad iQ™4 SO Has to be pipetted into PCR tubes recommended by manufacturer.
Bio Rad iQ™5 SO Has to be pipetted into PCR tubes recommended by manufacturer.
Bio Rad MyiQ™ SO Has to be pipetted into PCR tubes recommended by manufacturer.
Bio Rad MyiQ2™ SO Has to be pipetted into PCR tubes recommended by manufacturer.
DNA Technology DTlite TM
Eppendorf Mastercycler® EP Realplex TM
Qiagen Rotor-Gene Q SO Has to be pipetted into PCR tubes recommended by manufacturer.
Roche Light Cycler® 96 SO Adaptor and Calibration file required.
Roche Light Cycler® 480 SO Adaptor for instrument required, older instrument version requires calibration file.
Roche Light Cycler® Nano SO
Sacace SaCycler-96 TM
Techne® PrimeQ TM
Thermo Fisher Scientific PikoReal™ SO Has to be pipetted into PCR tubes recommended by manufacturer.
Thermo Fisher Scientific ProFlex™ TM
IT-IS Life Science Ltd MyGo! SO
IT-IS Life Science Ltd MyGo! Mini SO Has to be pipetted into PCR tubes recommended by manufacturer.

Specific and Precise

All product relevant microorganisms are detected within one single test. The detection limit is some 100 microorganisms which cannot be reached with any other method in the same time. Bacteria or yeasts from a sample are classified according to their spoilage potential and identified.

Safe and Robust

Our PCR kits are containing all components which are necessary for the analysis pre-dosed and dried in the PCR tubes. So it is guaranteed that all components are always included in the correct ratio in each test. We ship at ambient temperature, PIKA’s PCR kits are stable even at room temperature.

Fast and Cost Efficient

Processing of samples takes only 15 minutes. The results from thermocycling are available within 90 minutes. Our PCR kits are matching to each other. This allows that different analyses as detection of bacteria and yeasts may be done at the same time, efficient and cost saving.

PCR Detection Kits B – Detection and Identification of Bacteria

PIKA Detection Kits B were developed for the routine quality control of products and the production line. Different groups of microorganisms which are spoiling a product are specifically detected together by screening kits. A simple yes/no analysis covering all spoilage bacteria in one single test allows the fast and reliable control of the whole production line – and even the costs stay within reasonable limits. Further differentiation is achieved by identification kits which are available for a variety of bacteria species.

Available Kits:

  • LP Screening beer spoiling lactobacilli and pediococci
  • Alicyclobacillus sp. Screening
  • Lactic acid bacteria Screening
  • Acetic acid bacteria Screening
  • E. coli/coliforms Screening
  • Salmonella enterica Screening
  • LP single identification assays for all beer spoiling lactobacilli and pediococci
  • Identification of a diversity of other product spoiling bacteria species (Alicyclobacillus acidoterrestris, Lactobacillus species, Megasphaera, Pectinatus)

Fields of application:

  • Routine control in breweries
  • Identification and verification of positive
  • Routine control in juice production
  • Analysis of apple juice and Cider according to IFU No 12- the total analysis time is shortened 5 days
  • Monitoring of microbiological status

PCR Detection Kits H – Detection and Identification of Yeasts

PCR detection Kits H are optimized for the examination of beer and sugar containing food and beverages. The complete spectrum of yeasts and molds can be tested with one single analysis – free of yeasts and molds yes/no? Different yeasts which may be critical in different products as beer and wine are detected with specific screening or identification kits.

Available Kits:

  • Screening yeasts and molds
  • Brettanomyces/Dekkera Screening
  • Saccharomyces cerevisiae var. diastaticus identification
  • Dekkera bruxellensis identification
  • Dekkera custersianus identification

Fields of application:

  • Monitoring of microbiological status in all areas
  • Routine control and quantification in breweries
  • Routine control and quantification in wineries
  • Identification and verification of positives
  • Routine control of concentrates and sugar containing products

PCR Detection Kits S – Detection and Identification of Molds

PIKA Detection Kits S were developed for easy differentiation of molds. If molds are appearing in a process step or in the finished product, the question about their heat resistance is crucial for the further processing. With PCR detection kits, heat resistant molds are clearly identified. Our kits are also in use for the detection of mycotoxin producing Fusarium species on malt, grain and cereals as well as in flour.

Available Kits:

  • Byssochlamys sp. screening
  • Neosartorya sp. screening
  • HRM (heat resistant molds) screening
  • Fusarium sp. screening
  • Penicillium roqueforti group screening
  • Screening yeasts and molds

Fields of application:

  • Monitoring of microbiological status in all areas
  • Analysis of raw materials and concentrates
  • Routine control of fruit juices and sugar containing products
  • Identification and verification of positives
  • Analysis of malt, grain, grain products and flour

Further PIKA Products and Services

Competence in microbiology with a broad background: The innovative products from PIKA are derived from applications of our own in-house analytics and services for producers. All our products from the whole portfolio are perfectly matched. PIKA products are outstanding in easy handling and extreme reliability, our services in short processing time and top quality at affordable prices.

FastOrange Enrichment Media

Developed by us to avoid microbiological damage of beer and many other beverages: PIKA FastOrange can be used by every brewer and every beverage producer for routine  control throughout the whole process line. FastOrange is available in different varieties, according to the capabilities and equipment of your own lab.

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DNA Isolation Kits

Developed for use in a production laboratory: Optimised for the extraction of genomic DNA from beer, wine and other beverages as well as from malt, grain and cereals – for the detection of bacteria, yeasts and molds. Cost saving thanks to simple and fast handling and a minimum of equipment needs.

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DNA Analyses with PCR

Spoilt product or positive enrichments are further discriminated – fast and specific by PCR analyses the same day as the samples are received. We further identify unknown bacteria, yeasts or molds from product or the production area within 3 to 5 days by DNA sequencing.

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Broad Process Consultancy

Our work does not end at the analysis result, we further advise you how to deal with a contamination and propose the next steps to minimize the risk for further batches in your production line. You can also count on us when optimizing your routine quality control measures.

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PIKA Weihenstephan – your partner in microbiology for beverage production
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